Journal: CytoJournal

Article Title: The mechanism of prostaglandin E2 upregulation of programmed death ligand 1 expression promoting immune escape in non-small cell lung cancer

doi: 10.25259/Cytojournal_129_2025

Figure Lengend Snippet: PGE2 upregulates PD-L1 expression in NSCLC and promotes immune escape response. (a-c) PD-L1 expression detected after PTGES overexpression (OE-PTGES) and knockdown (sh-PTGES), compared with respective negative controls (OE-NC or sh-NC). (d and e) Cytotoxicity tested by LDH kit assay after PTGES overexpression (OE-PTGES) and knockdown (sh-PTGES), compared with respective negative controls (OE-NC or sh-NC). (f and g) CD8 + T cell viability tested after PTGES overexpression (OE-PTGES) and knockdown (sh-PTGES), compared with respective negative controls (OE-NC or sh-NC). (h and i) CD8 + T cell apoptosis examined after PTGES overexpression (OE-PTGES) and knockdown (sh-PTGES), compared with respective negative controls (OE-NC or sh-NC). (j-m) IFN-γ, TNF-α, granzyme B, and perforin quantification by ELISA after PTGES overexpression (OE-PTGES) and knockdown (sh-PTGES), compared with respective negative controls (OE-NC or sh-NC). n = 6; ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001. PEG2: Prostaglandin E2, PD-L1: Programmed death ligand 1, NSCLC: Non-small cell lung cancer, PTGES: Prostaglandin E synthase, OE-NC: Overexpression negative control, sh-NC: Short hairpin negative control, LDH: Lactate dehydrogenase, OE-PTGES: Overexpression prostaglandin E synthase, sh-PTGES: Short hairpin prostaglandin E synthase, IFN-γ: Interferon-gamma, TNF-α: Tumor necrosis factor-alpha, ELISA: Enzyme-linked immunosorbent assay.

Article Snippet: First, a single-cell suspension was prepared and incubated with CD3 (E-AB-F1013E, Elabscience, Wuhan, China) and CD8 (E-AB-F1104Q, Elabscience, Wuhan, China) antibodies in the dark.

Techniques: Expressing, Over Expression, Knockdown, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: CytoJournal

Article Title: The mechanism of prostaglandin E2 upregulation of programmed death ligand 1 expression promoting immune escape in non-small cell lung cancer

doi: 10.25259/Cytojournal_129_2025

Figure Lengend Snippet: PGE2 promotes immune escape in NSCLC in vivo by upregulating PD-L1 expression. (a) Isolated tumor images after PTGES overexpression and knockout. (b and c) Changes in tumor weight and volume after PTGES overexpression and knockout (significant difference markers marked with ✶ represent OE-NC versus OE-PTGES, and those marked with # represent sh-NC vs. sh-PTGES). (d-f) WB analysis of PTGES and PD-L1 after PTGES overexpression and knockout in vivo . (g and h) IHC analysis of CD8 after PTGES overexpression and knockout in vivo (scale bar: 20 μm, magnification, 400×). (i-l) IFN-γ, TNF-α, granzyme B, and perforin quantification by ELISA after PTGES overexpression and knockout in vivo . n = 5; ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001, ## P < 0.01. OE-NC: Overexpression negative control, sh-NC: Short hairpin negative control. PEG2: Prostaglandin E2, PD-L1: Programmed death ligand 1, NSCLC: Non-small cell lung cancer, PTGES: Prostaglandin E synthase, OE-NC: Overexpression negative control, sh-NC: Short hairpin negative control, OE-PTGES: Overexpression prostaglandin E synthase, sh-PTGES: Short hairpin prostaglandin E synthase, IHC: Immunohistochemistry, IFN-γ: Interferon-gamma, TNF-α: Tumor necrosis factor-alpha, ELISA: Enzyme-linked immunosorbent assay.

Article Snippet: First, a single-cell suspension was prepared and incubated with CD3 (E-AB-F1013E, Elabscience, Wuhan, China) and CD8 (E-AB-F1104Q, Elabscience, Wuhan, China) antibodies in the dark.

Techniques: In Vivo, Expressing, Isolation, Over Expression, Knock-Out, Enzyme-linked Immunosorbent Assay, Negative Control, Immunohistochemistry